File manipulation & speed

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Hallo to all.
A simple question.
If i open 2 files outside my main and close the same at the end of my program....if i call into my main (many many time)
the function "seekfile" "readfloat" (and others too...)i have a speed down of my program?
And if yes....i have to open the file,read the data,close the file ALL outside my main?
Thanks.